Formation of mSOD1 high molecular mass species is regulated by COMMD1.

<p><b>A.</b> HEK293T cells were co-transfected with SOD1-Flag mutants (A4V, G37R, G85R, D90A, G93A, E100G) in combination with EV (–) or HA-COMMD1 prior to lysis and detection of SOD1 multimeric complexes using SDS-PAGE under non-reducing conditions (– β-ME; 40 μg). Immunoblotting was performed using antibodies directed against the Flag-tag and HA-COMMD1 proteins. Input samples were analysed in presence of β-ME (+ β-ME; 30 μg). Samples were analysed on separated membranes. <b>B.</b> HEK293T cells were co-transfected with SOD1-Flag WT in combination with EV (–) or HA-COMMD1 prior to lysis and analysed as described in Figure 3A. <b>C.</b> Visualization of SOD1 HMW complexes in Neuro2A cells transfected with SOD1-Flag mutants G85R and G93A in combination with EV (–) or HA-COMMD1 as described in Figure 3A. Samples were analysed on separated membranes.