Enhanced EMT gene changes in <i>NRF2</i>-knockdown HK2 cells.

<p>(A) Transcript levels of <i>NRF2</i> in unmodified HK2 cells, nonspecific scRNA-expressing HK2 (sc) cells, and NRF2 shRNA-expressing HK2 (shNRF2) cells. (B) ARE-driven luciferase activity was measured in the sc and shNRF2 HK2 cells. ^aP<0.05 compared with the sc cells. (C) The sc and shNRF2 HK2 cells were incubated with TGFβ1 (10 ng/ml) for 48 h and the transcript levels of EMT genes were determined. The values are ratios with respect to each vehicle (veh)-treated sc control and are the means ± SE of 3 experiments. ^aP<0.05 compared with the TGFβ1-treated sc cells.