Caspase-2 is induced in osteoclasts on treatment with oxidative stress-inducing agents.

<p>(A) Primary cultures of <i>Casp2^+/+</i> osteoclasts treated with 500 μM H₂O₂ for the indicated time points (n = 3), were fixed and probed for caspase-2 (green); nuclei were stained blue. <i>Casp2^−/−</i> osteoclasts treated with 500 μM H₂O₂ for 4 h served as a negative control. Scale bar, 50 μm. (B) RAW 264.7 macrophages were differentiated into osteoclasts, treated with 500 μM H₂O₂ for the indicated time periods, lysed and probed for caspase-2 (n = 3). Stripped blot was probed for total actin as loading control.