Differential gene expression profiles in white, gray, and opaque cells.

<p>Differentially expressed genes were defined as ones with greater than or equal to 4-fold difference of relative expression levels revealed by RNA-Seq analysis between two different cell types. The numbers of overlapping genes less than 4-fold difference of relative expression levels are also show. (A) Distinct gene expression patterns of white-gray, white-opaque, and gray-opaque cell types. The numbers indicate differentially expressed genes between the two cell types compared. (B) Distinct gene expression patterns of white, gray, and opaque cell types. The numbers indicate white (69)-, gray (45)-, or opaque (129)-enriched genes. (C) Verification of cell type-enriched genes by quantitative real-time PCR assays. <i>WH11</i>, white-enriched; <i>OP4</i> and <i>WOR1</i>, opaque-enriched; <i>HSP31</i>, <i>LIP9</i>, <i>HMS1</i>, <i>CAS2</i>, and <i>PGA26</i>, gray-enriched.