cFLIP is essential for HG-induced cell growth in rat MCs.

<p>(<b>a</b>) In normal-glucose condition, over-express of cFLIP promotes proliferation and Fibronectin synthesis, and inhibit apoptosis via blocking both extrinsic and intrinsic apoptosis pathway. The increased proliferation and Fibronectin synthesis can't be suppressed by by 50 μM emodin. (<b>b</b>) MTT assay shows over-express of cFLIP in normal-glucose medium can increase cell viability. (<b>c, d</b>) Flow cytometry analysis indicates that over-express of cFLIP in normal-glucose medium decrease G0–G1 phase and cell death, and increases S phase. (<b>e</b>) Western blotting reveals that knockdown of cFLIP using shRNA strategy can suppress proliferation and Fibronectin synthesis, and promote apoptosis via both extrinsic and intrinsic apoptosis pathway. (<b>f</b>) MTT assay shows knockdown of cFLIP decreases cell viability. (<b>g, h</b>) Flow cytometry analysis indicates that knockdown of cFLIP increases G0–G1 phase and cell death, and decreases S phase. *P<0.05 vs. scramble.