The expression level of MTs in mice liver at 3-week and 35-week.

<p>A-B: Real-time RT-PCR analysis of MT-1/2 gene expression. Both the Maotai +DEN group and ethanol+ DEN group produced a significant induction of MT-1 in mRNA level at 3-week. But Maotai +DEN group still produced a dramatic induction of MT-1/MT-2 at 35-week, it was about 19 times of ethanol+ DEN group in MT-1 (<i>p</i><0.01). C–D: Western blot analysis of MTs protein level at 3-week and 35-week. lane 1.Control; lane 2.Maotai; lane 3.Ethanol; lane 4.Maotai+DEN; lane 5.Ethanol+DEN; lane 6.DEN; E: Quantification of MTs level by western blot analysis. The specific up-regulation of MT-1/2 was confirmed through western blotting assay. Maotai+ DEN always had a higher MTs protein level. but it was generally lower in ethanol+ DEN group. F-H, I-K: Light microscopy of MTs expression through Immunohistochemistry in control group, Maotai+ DEN group and ethanol+ DEN group at 3-week and 35-week (400× magnification, allows indicate MTs expression cytolymph and nucleus positive cells); L: Quantification of MTs expression in Immunohistochemistry. Immunohistochemical assay also showed similar MTs protein expression with western blotting assay (<i>p</i><0.01). *Significantly different in Maotai+ DEN group and ethanol+ DEN group from control group, ^#Significantly different in Maotai+ DEN group from ethanol+ DEN group, <i>p</i><0.05.