The relationship between apparent cell associated concentrations of indole and supernatant concentrations of indole.

<p>Known concentrations of indole were added to the supernatant of stationary phase BW25113 Δ <i>tnaA</i> cells, the mixture was vortexed and centrifuged, and the resultant pellet assayed for indole using Kovacs assay. Dotted lines indicates the apparent cell associated indole at the peak of the pulse (60 mM) and the corresponding supernatant concentration and indicates the apparent cell associated indole when cells are in stationary phase and the corresponding supernatant concentration (0.75 mM). Data shown are the mean values ± standard deviation for at least three independent repeats.