The areas of the amygdala sampled and the distributions of ACIII immunopositive cilia in subdivisions of amygdala in WT and Bbs4^-/- mice are illustrated.

<p>Figure A shows the subdivisions of amygdala and the coronal plane that was analyzed in this study. Figures B and C are maps documenting distribution of cilia within these regions of WT (B) and Bbs4^-/- (C) brains that were acquired at high magnification using Sterioinvestigator image analysis software. Figure D shows the density of cilia ± the SEM within each amygdala subdivision of WT (blue) and Bbs4^-/- (gray) animals; asterisks and brackets indicate where statistical analysis demonstrated statistical differences between WT and Bbs4^-/- mice. The data for (D) was collected from three WT and five Bbs4^-/- mice. High magnification comparisons of the distribution of cilia within comparable regions of each of the amygdala subdivisions are demonstrated in figures E through N. Note the association of cilia with neurons counterstained with neutral red in each region as well as the dramatic reduction in ACIII+ cilia in Bbs4^-/- animals. BLA = basolateral nucleus of amygdala; BMa = basomedial amygdala, anterior part; BMp = basomedial amygdala, posterior part; CeA = central nucleus of amygdala with capsular (c), lateral (l) and medial (m) subdivisions; ICM = intercalated cell masses; LA = lateral nucleus of amygdala. Scale bar in figure A = 1 mm, in N = 20 μm; figures E – N are of the same magnification.