Hydroxyl radical-scavenging activities in hair follicles determined by an electron spin trapping ESR assay.

<p>The effects of equal amounts of each minced tissue sample on hydroxyl radical (⋅OH) generation in the Fenton reaction was studied using the ESR method, as described in the text. (A) Representative ESR spectra of DMPO-⋅OH with the hair bulb and mid-segment tissue samples. Hydroxyl radicals were generated by the Fenton reaction (DMPO: 400 mM). (B) Histogram showing hydroxyl radical-scavenging activity, which are expressed as means ± SD of 3 independent experiments, *<i>P</i><0.05, compared to pigmented hair follicles.