gga-miR-375 inhibited DF-1 cell proliferation and invasion.

<p>The cells transfected with gga-miR-375, miR-NC, or mock were subjected toWST-1 analysis, colony formation, and wound healing assay. (<b>A</b>) Effects of gga-miR-375 on proliferation over different time periods. Plotted means and standard errors were computed from data of three independent experiments; bars, SEM. **<i>P</i><0.01. (<b>B</b>) Effects of gga-miR-375 on colony formation of DF-1 cells. (<b>C</b>) Images of cell migration from wound healing assay. Scratch wounds were made on confluent monolayer cultures 48 hours post transfection. Images of wound repair were taken at 0, 24, and 48 hours after wound. (D)The percentage of wound closure was normalized to the wound area at hour 0 (above panel). Plotted means and standard errors were computed from data of three independent experiments. The comparisons were evaluated using t-test; bars, SEM. *<i>P</i><0.05.