IGFBP-3 NB restores insulin receptor phosphorylation, while reducing IRS-1^Ser307 phosphorylation.

<p>For all studies, groups are control (ctrl), diabetic (Diabetic), Diabetic +IGFBP-3 NB (Diab +BP3), Diabetic +CMV (Diab+CMV), Diabetic+IGFBP-3 NB left eye (Diab+BP3 contra) and Diabetic+CMV left eye (Diab+CMV Contra). Panel A presents Western blot results for the ratio of phosphorylated insulin receptor tyrosine 1150/1151 to total insulin receptor. Panel B shows the ratio of phosphorylated IRS-1 on serine 307 to total IRS-1. IRS-1^Ser307 is inhibitory to insulin signal transduction. *P<0.05 vs ctrl. #P<0.05 vs. Diabetic only, $P<0.05 vs. Diab+BP3. N = 5 for all groups. Data are mean ± SEM.