Mizoribine suppresses aldosterone-induced renal pyroptosis.

<p>Aldosterone (ALD) enhanced the renal expression of the nucleotide-binding oligomerization domain, leucine-rich repeat and pyrin domain containing (NLRP) 3 and caspase-1, and increased the number of TUNEL-positive cells—the hallmark of pyroptosis—whereas the increase in these factors was significantly suppressed by mizoribine (MZR). (A) Western blot analysis for NLRP3; caspase-1; and α-tubulin was performed. The total cell lysate was prepared from the rat kidney cortex. Graphs show the expression level quantified by densitometry and normalized with α-tubulin. (B) Representative photomicrographs of TUNEL staining of kidney sections. The graph shows the quantification of TUNEL-positive cells. Values are presented as mean ± SEM. *<i>P</i><0.05.