KSHV v-cyclin can partially rescue MHV68 v-cyclin null virus replication defect in growth arrested RL-65 cells.

<p>(A) Schematic illustration depicting the v-cyclin locus and insertion of KSHV v-cyclin in place of the MHV68 v-cyclin open reading frame. (B) RL-65 cells were plated to confluency and then infected at an MOI of 0.05 with either WT MHV68, vCyclin.stop or a recombinant MHV68 expressing the KSHV v-cyclin from the MHV68 v-cyclin locus (K-cyclin). Cells plus supernatants were collected every 24 hours and subjected to freeze/thaw twice to release any intracellular virus. Total virus at each time point was determined by plaque assays on NIH 3T12 fibroblasts. The data shown was compiled from 3 separate experiments, each done in triplicate. Standard error of the mean is shown.