Distribution of channelrhodopsin 2-Venus conjugates (ChR2V) in the trigeminal ganglion (TG) neurons.

<p><b>A-C</b>, Immunohistochemical identification of ChR2V-positive (ChR2V+) neurons using cell-type specific markers, NF200 (A), CGRP (B) and P2X₃ (C). Scale bars indicate 20 μm. <b>D</b>, Probability of the co-expression of each marker, NF200, CGRP or P2X₃, in the ChR2V+ neurons. <b>E</b>, Probability of the co-expression of ChR2V in the neurons positive for each marker, NF200, CGRP or P2X₃. <b>F</b>, The average diameters of the TG neurons were compared among four groups positive for ChR2V (green), NF200 (blue), CGRP (red) and P2X₃ (orange).