Sequence alignment and experimental confirmation of viral identity.

<p>Panel A: 50 bp reads mapping to the HSV1 (strain F, gi: 290766003) genome and measles virus (Edmonston strain, gi: 331784) genome are shown. Panel B: Virus–specific PCR amplicons are shown with the positive control (viral DNA: HSV 17 syn+, clinical strain VZV, MV extracted from vaccine) and negative controls (ddH₂O). Custom primers were required to amplify HSV1 from specimen 924, confirmed by amplicon sequencing (data not shown).