<p>(<b>A, B</b>) LSK were gated with c-kit and Sca-1 in BM (<b>A</b>) and spleen (<b>B</b>). The percentages of LSK are shown(right panel in A and B). CD150<sup>+</sup> CD48<sup>−</sup> HSC in BM (<b>C</b>) and spleen (<b>D</b>) are given. Closed and open bars indicate WT and JAK2V617F individual mice, respectively. The data are representative of those obtained in two independent experiments (N = 8, 4) (<b>E</b>) Distinct HSC were resolved as EPCR<sup>+</sup> CD45<sup>+</sup> CD150<sup>+</sup> and CD48<sup>−</sup>. The percentages of CD45<sup>+</sup> EPCR<sup>+</sup> CD150<sup>+</sup> CD48<sup>−</sup> in 10<sup>6</sup> BM were increased in JAK2V617F mice. The data are representative of those obtained in two independent experiments (N = 6). ...
<p>(<b>A</b>) Splenic cDCs were gated as MHCII<sup>hi</sup>CD11c<sup>hi</sup> cells (top panel) and ...
<p>Spleen (SPL) (<b>A</b>) and skin draining lymph nodes (SDLN) (<b>B</b>) of 8 weeks old WT and DKO...
<p>A. Brightfield microscopy of representative spleen sections from day 9 and 30 sRBC immunized WT a...
<p>(<b>A</b>) LSK were resolved with CD150 and CD34. CD34<sup>−</sup> CD150<sup>Hi</sup>, <sup>Lo</s...
<p>Representative flow cytometric analysis for Lin<sup>−</sup> bone marrow cells for Sca-1<sup>+</su...
<p>(A and B) Frequency (A) and number (B) of Ki67<sup>+</sup> within HSPCs populations in BM of naïv...
<p>(<b>A</b>) LSK from control and Tg mice were resolved with CD150, CD48, Flk2 and CD34 staining in...
<p>(<b>A</b>) The LSK<sup>−</sup> cell population is basically CD150-negative. LSK<sup>+</sup> (Lin<...
<p>(A) Gating strategies. (B) FC plots of FL-HA binding versus CD44 expression by BM LSK, CLP, GMP, ...
<p>(<b>A</b>-<b>C</b>) Single cell suspension was isolated from non-treated wild type mice, 3, or 7 ...
<p>(A) Representative FACS profiles of lineage-, c-Kit+ and Sca1+ cells separated based on CD34 and ...
<p>(A) 50, 100 or 200 HSCs sorted from <i>Prep1<sup>+/+</sup></i> and <i>Prep1<sup>i/i</sup></i> FLs...
<p>(A) TCRβ and B220 (upper panels), and CD4 and CD8 (lower panels, gated on TCRβ<sup>+</sup> cells)...
<p>(A) Plots depict CD44 and CD62L expression on splenic <i>Hdac1<sup>f/f</sup></i> and <i>Hdac1<sup...
Mice were infected A) Frequency and number of cDCs (CD11c+ PDCA-1−) and pDCs (CD11clow PDCA-1+) iden...
<p>(<b>A</b>) Splenic cDCs were gated as MHCII<sup>hi</sup>CD11c<sup>hi</sup> cells (top panel) and ...
<p>Spleen (SPL) (<b>A</b>) and skin draining lymph nodes (SDLN) (<b>B</b>) of 8 weeks old WT and DKO...
<p>A. Brightfield microscopy of representative spleen sections from day 9 and 30 sRBC immunized WT a...
<p>(<b>A</b>) LSK were resolved with CD150 and CD34. CD34<sup>−</sup> CD150<sup>Hi</sup>, <sup>Lo</s...
<p>Representative flow cytometric analysis for Lin<sup>−</sup> bone marrow cells for Sca-1<sup>+</su...
<p>(A and B) Frequency (A) and number (B) of Ki67<sup>+</sup> within HSPCs populations in BM of naïv...
<p>(<b>A</b>) LSK from control and Tg mice were resolved with CD150, CD48, Flk2 and CD34 staining in...
<p>(<b>A</b>) The LSK<sup>−</sup> cell population is basically CD150-negative. LSK<sup>+</sup> (Lin<...
<p>(A) Gating strategies. (B) FC plots of FL-HA binding versus CD44 expression by BM LSK, CLP, GMP, ...
<p>(<b>A</b>-<b>C</b>) Single cell suspension was isolated from non-treated wild type mice, 3, or 7 ...
<p>(A) Representative FACS profiles of lineage-, c-Kit+ and Sca1+ cells separated based on CD34 and ...
<p>(A) 50, 100 or 200 HSCs sorted from <i>Prep1<sup>+/+</sup></i> and <i>Prep1<sup>i/i</sup></i> FLs...
<p>(A) TCRβ and B220 (upper panels), and CD4 and CD8 (lower panels, gated on TCRβ<sup>+</sup> cells)...
<p>(A) Plots depict CD44 and CD62L expression on splenic <i>Hdac1<sup>f/f</sup></i> and <i>Hdac1<sup...
Mice were infected A) Frequency and number of cDCs (CD11c+ PDCA-1−) and pDCs (CD11clow PDCA-1+) iden...
<p>(<b>A</b>) Splenic cDCs were gated as MHCII<sup>hi</sup>CD11c<sup>hi</sup> cells (top panel) and ...
<p>Spleen (SPL) (<b>A</b>) and skin draining lymph nodes (SDLN) (<b>B</b>) of 8 weeks old WT and DKO...
<p>A. Brightfield microscopy of representative spleen sections from day 9 and 30 sRBC immunized WT a...