Particular HSC subsets expand in JAK2V617F Tg mice.

<p>(<b>A</b>) LSK were resolved with CD150 and CD34. CD34⁻ CD150^Hi, ^Lo, and negative HSC were gated as described by Morita <a href="http://www.plosone.org/article/info:doi/10.1371/journal.pone.0093643#pone.0093643-Morita1" target="_blank">[10]</a>. The percentages of CD34⁻ HSC subsets in BM were calculated. Closed and open circles indicate individual WT and JAK2V617F mice, respectively. The data are representative of those obtained in two independent experiments (N = 6). (<b>B</b>) Lower side population cells in BM (spindle-shaped gating) are thought to include myeloid biased HSC. The “side population tip” was increased in JAK2V617F mice. Representative plots are shown in the left panels, while cell percentages of lower and upper cells are given in right panels. (<b>C</b>) Both CD86⁻ and CD86⁺ CD150⁺ CD48⁻ LSK cells in BM were increased in JAK2V617F mice. CD48⁻ LSK were resolved with CD86 and CD150. Cell percentages of CD86⁻ and CD86⁺ in CD150^Hi were calculated. The data are representative of those obtained in three independent experiments (N = 8) <i>p</i><0.01(**), <i>p</i><0.05(*).