GCRs retaining <i>hph</i> belong to two size classes.

<p>(<b>A</b>) Digestion of the uGCR chrV divides the uGCR chrV into left telomeric, internal, and right telomeric fragments. Vertical arrows indicate the <i>Asc</i>I cleavage sites and relevant chromosomal features are labeled. (<b>B</b>) Southern blot using an <i>hph</i> probe of a pulsed-field gel (PFG) with DNA from the wild-type strain (RDKY6677) and 6 GCR-containing isolates (212, 214, 215, 217, 218, and 219) with and without <i>Asc</i>I digestion. The <i>hph</i> probe hybridizes to the intact chromosome and the internal and left telomeric fragments. (<b>C</b>) Southern blot of a second PFG with the same samples as in panel B using an <i>MCM3</i> probe. The <i>MCM3</i> probe hybridizes to the intact chromosome and the internal fragment.