Systematic Evolution of Ligands by Exponential Enrichment (SELEX).

<p>DNA was incubated with immobilized rh E-selectin on magnetic beads (target beads). After washing and removing unbound DNA, target bound DNA was eluted and amplified via PCR. The double-stranded PCR product was separated in single strand DNA and the next SELEX step started after this regeneration. The identification of aptamers via cloning and sequencing of the enriched ssDNA pool was performed after several SELEX rounds (10–20 rounds).