Genetic dissection narrows down <i>Toxo1</i> to 0.89 megabase.

<p>(<b>A</b>) Genetic dissection based on <i>in vitro</i> phenotypes. [³H] uracil incorporation into <i>T. gondii</i> RNA as a read-out of parasite proliferation within macrophages <i>in vitro</i>; results are normalized according to the values obtained in BN macrophages. Columns and bars show mean ± SD, n = 4 except LEW (n = 8) and BN.LEWc10-Cf (n = 3); _*: <i>p</i><0.05; _**: <i>p</i><0.01 as compared to BN). (<b>B–D</b>) Genetic dissection based on <i>in vivo</i> phenotypes. (<b>B</b>) Anti-<i>T. gondii</i> IgG response and cyst number were analyzed in BN, LEW and six congenic BN.LEWc10 lines. Anti-<i>T. gondii</i> IgG Ab response was analyzed by ELISA at day 30 post infection. (<b>C</b>) Number of brain cysts was determined by epifluorescence at day 60. (<b>C–D</b>) Columns and bars show mean ± s.e.m, n = 5 except for BN.LEWc10-Ce and Cga (n = 4); _*:<i>p</i><0.05; _**:<i>p</i><0.01 (as compared to BN). (<b>D</b>) Genotypes at the markers of the <i>Toxo1</i> locus in the used BN.LEWc10 congenic lines. The grey zone indicates the <i>Toxo1</i> locus narrowed down to 0.89 Mb (boundary markers: D10GF49 and D10GF55; physical distances are indicated between markers in kilobases).