Morphologic changes, identification and growth of gMDSCs.

<p>Cell suspensions from skeletal muscle tissues were found to contain large numbers of small and rounded satellite cells. The vast majority of cells first transformed into spindle-shaped mononuclear cells after the second passage. Positive desmin, α-sarcomeric actinin, MyoD1, Myf5, and PAX7 immunostaining was visualized as green, whereas DAPI-positive nuclei were stained blue. gMDSCs and gMDSCs–pDsRed2-1 entered an exponential growth phase between days 2 and 3, and reached a growth plateau between days 6 and 7. gADSCs and gADSCs–pDsRed2-1 gave results similar to those for gMDSCs and gMDSCs–pDsRed2-1. gMDSCs, goat muscle-derived satellite cells; gADSCs, goat adipose-derived mesenchymal stem cells.