Defective gas exchange and respiratory failure following influenza virus infection in <i>Csf2</i>^−/− mice lacking AM.

<p>WT and <i>Csf2</i>^−/− mice were infected i.t. with 50 pfu PR8 influenza virus. (A) Panels show H&E-stained histological lung sections of day 10-infected WT and <i>Csf2</i>^−/− mice. The concentration of total protein (B) and cholesterol (C) in the BAL was determined at the indicated time points after infection. (D) Percentages of dead cells (eFluor780⁺) and debris (eFluor780⁻CD45⁻) in the BAL were determined by flow cytometry at d6 and d10. Bar graphs show the total BAL event number (E) and numbers of eFluor780⁺ or eFluor780⁻CD45⁻ events (F). Shown is the mean ± SD of 4–5 mice per group. Arterial blood oxygen saturation (G) and O₂ partial pressure (H) was measured in infected animals at day 9. Symbols represent values of individual mice and the mean is shown.