DBA/2J.BALB<i>^Rgcs1</i> substrain mice have more severe glaucomatous damage.

<p>(A) Intraocular pressures (IOP) of eyes from 3 month and 10 month DBA/2J wild type and substrain mice. Mice homozygous for the BALB/cByJ allele of <i>Rgcs1</i> (CC) exhibit no difference in IOPs at either age (P = 0.275, 3 months; P = 0.889, 10 months) compared to mice carrying at least one DBA/2J allele for <i>Rgcs1</i> (DD and DC, respectively). For CC mice data was collected from 15 and 42 eyes at 3 months and 10 months, respectively. For DC mice, data was collected from 28 and 41 eyes, respectively. For DD mice, data was collected from 20 and 136 eyes, respectively. (B) Bar graph showing the frequency of mild, moderate, and severe optic nerve damage in 10 month old DBA/2J mice carrying either the D or C alleles for <i>Rgcs1</i>. Mice homozygous for the BALB/c allele (CC) have a higher frequency of severe optic nerve damage than mice with the D allele (β² P = 0.013, CC compared to the combined DD and DC animals). Nerves from 163, 90, and 40 DD, DC, and CC mice, respectively were scored. (C) Images of whole mounted retinas, surface stained with DAPI showing exemplars of mild, moderate, and severe damage. (D) Bar graph showing the mean retinal cell density of 10 month old DBA/2J mice. (*P<0.001, CC compared to DD and DC samples combined).