H3K4me3 redistribution from TSS to miRNA-coding region affects miRNA expression in LPS-conditioned moDCs.

<p>(<b>A</b>) Proportion of H3K4me3 redistribution around the TSSs in the LPS- (LPS) or TGF-β(TGFβ)-upregulated miRNA with H3K4me3 modification. (<b>B</b>) H3K4me3 redistribution around the TSSs in the LPS-upregulated miRNA with H3K4me3 modification. For ChIP-Seq analysis, the MoDCs were exposed to LPS (100 ng/ml) for 24 hrs. The gene structure was downloaded from the UCSC Genome Brower. Enriched regions were found in the UCSC Genome Browser (<a href="http://genome.ucsc.edu" target="_blank">http://genome.ucsc.edu</a>.). Histone modification peaks were detected by CHIPOTle. The miRNA positions were predicted by Ensembl. The definitive and putative miRNATSSs were inferred from <a href="http://mirstart.mbc.nctu.edu.tw/browse.php" target="_blank">http://mirstart.mbc.nctu.edu.tw/browse.php</a>. (<b>C</b>) qRT-PCR analysis of microRNA in LPS-conditioned DCs. moDCs were exposed to LPS (100 ng/ml) for 24 hrs, and the total RNAs was extracted from LPS-conditioned DCs. R.E, relative expression. The qRT-PCR data were representative of three healthy donors. The arrow points in the direction of gene transcription. *, P<0.05; **, P<0.01.