IL-10 regulates the frequencies of CD4⁺ and CD8⁺ T cells expressing IL-19 and IL-24 and modulates IL-19 and IL-24 gene expression in filarial infections.

<p>(A) The frequencies of CD4⁺ and CD8⁺ T cells expressing IL-19, IL-24 and IL-26 following IL-10 neutralization and stimulation with BmA in a subset of INF individuals (n = 7). (B) The frequencies of CD4⁺ and CD8⁺ T cells expressing IL-19, IL-24 and IL-26 following addition of rhIL-10 and stimulation with BmA in a subset of INF individuals (n = 7). Antigen – stimulated frequencies are shown as net frequencies with the baseline levels subtracted. (C) PBMCs from infected (INF) individuals (<i>n</i> = 7) were cultured with BmA alone or BmA with rhIL-10 for 24 h and mRNA expression of IL-19, IL-24 and 18s RNA were measured by RT-PCR. Data are shown as fold change BmA plus IL-10 over BmA alone controls. Each line represents a single individual. P values were calculated using the Wilcoxon signed rank test.