DH-PS induced IL-1ra production in human PBMC, monocytes, but not neutrophils.

<p>(A) Human PBMCs were cultured (2×10⁶ cells/ml) with increasing concentrations of DH-PS for 18 hrs and the supernatants were harvested for IL-1ra measurements. (B) Human monocytes (CD14⁺ cells) were cultured (1×10⁶ cells/ml) with increasing concentrations of DH-PS for 18 hrs and the supernatants were harvested for IL-1ra measurements. (C) Neutrophils were cultured (1×10⁵ cells/ml) with the increasing concentrations of DH-PS for 3 or 24 hours and supernatants were harvested for IL-1ra measurements. Y-axis represented the mean concentration of IL-1ra (pg/ml). X-axis represented the concentration of DH-PS (μg/ml). Concentration 0 represented the use of PBS only as vehicle control. Results were presented as mean values with error bars showing the standard deviation of triplicate. Statistically significant difference: * compared with PBS-treated group, p<0.05. # compared with PBS-treated group, p<0.005.