Immunohistochemical localization of podocyte proteins in kidney tissues.

<p>The tissues were prepared with IVCT and immersion-fixation methods under various hemodynamic conditions. The micrographs of A, B, C, and D show the localization of integrin β1; F, G, H and I exhibit control stainings ommitting the integrin β1 primary antibody; J, K, L and M show the FAK localization; and O, P, Q, and R present the localization of p-Tyr397 FAK. T, U, V and W show the control stainings ommitting the FAK or p-Tyr397 FAK primary antibody. Immunohistochemical localizations of three proteins prepared with IVCT are shown under normotensive condition (A, J, O), acute hypertensive condition (B, K, P), and cardiac arrest condition (C, L, Q). The tissues in D, M, and R were treated by the immersion-fixation method. Figs. 2E, 2N, and 2S show the IODs of each protein under various hemodynamic conditions. N: normotension; AH: acute hypertension; CA: cardiac arrest; R: resected tissue. Scale bars = 20 μm. Histogram shows integral optical densities of each protein. IOD: integral optical densities. #: P<0.05.