Expression of MC4R mutants.

<p>A) ELISA of HEK293 cells expressing wild-type MC4R or mutations. The cell surface expression was normalized to total expression for each mutant and then to wild-type receptor for that batch (* denotes p<0.05 compared to wild-type by a one way ANOVA with Dunnet's post-hoc test). □ MC4R mutant D90N was not found in our cohort. B) Cell surface localization of HEK-293 cells expressing mutant or wild-type (BBS-MC4R) constructs labeled with Bungarotoxin-Texas Red. C) Normalized GFP loading reveals no differences in HA-MC4R mutant lifespan. HA-MC4R expression was normalized to GFP expression and plotted as a percentage of wild-type for each blot (n≥3).