Effect of proteasome or autophagy inhibitor on shRNA-mediated modification of Nhtt150Q-EGFP aggregation.

<p>Nhtt150Q-EGFP cells transfected with control (NSC) or shRNAs for indicated genes were treated with 0.5 μM MG132, 0.5 μM Baf A1 or DMSO together with ponasterone A for 24 hr. The cells with Nhtt150Q-EGFP aggregates were quantified by ArrayScan reader. Some of shRNA's suppressive effects on the aggregation were relieved by treatment with MG132 but not with Baf A1. Values are means ± SD of four well data (*<i>P</i><0.05, **<i>P</i><0.01, ***<i>P</i><0.001).