Global analysis of differentially expressed genes in response to Pi starvation or phoB inactivation in EDL933 strain.

<p><b>A.</b> Classification of genes whose expression levels were altered in Pi-dependent and in PhoB-dependent manners. Left and right circles indicate the differentially expressed genes of wild-type and Δ<i>phoB</i>-mutant strains with expression levels that were altered over 2-fold under Pi limitation. Venn diagram: Group 1 includes 936 PhoB-independent genes that are differentially expressed under Pi limitation in the wild-type strain, but that did not change in the Δ<i>phoB</i> mutant. Group 3 includes 472 PhoB-dependent genes differentially expressed in the Δ<i>phoB</i> mutant but did not change under Pi-limitation in the wild-type strain. Group 2 included 131 PhoB-dependent Pi response genes that are differentially expressed under Pi limitation in the wild-type strain and between the wild-type and the Δ<i>phoB</i> strains. <b>B.</b> Functional classification of genes with altered expression in strain EDL933 grown in Pi-limited conditions compared to cells grown in Pi-rich conditions (Pi-dependent (white bars)) and EDL933 incubated in Pi-limited conditions compared to Δ<i>phoB</i> mutant cells grown in the Pi-limited conditions (PhoB-dependent (gray bars)).