Telomere recombination measured by T-SCE is variably affected by <i>WRN</i> and/or <i>BRCA1</i> knockdown.

<p>ALT cell lines VA-13, Saos-2, and U-2 OS were transfected with <i>WRN</i>, <i>BRCA1</i>, or <i>WRN/BRCA1 siRNAs</i> and metaphase spreads were processed for T-SCE after 72 hours. Telomeres were fluorescently labeled and analyzed. <b>A</b>. A representative image of a metaphase spread from VA-13 cells shows DAPI-labeled chromosomes in blue and Cy-3 labeled telomeres in red. <b>B</b>. Results depict the frequency of T-SCE per chromosome analyzed from at least 30 metaphase spreads per treatment group. Western blots depict the ability of <i>BRCA1 siRNAs</i> to knock down BRCA1 in each cell line.