Phenotype and physiological characterization of <i>OsSWEET5</i>-overexpressing transgenic plants.

<p>(A) Photographs of WT and <i>OsSWEET5</i>-overexpressing lines (T₂) at 15 days after germination. The bar indicates 10 cm. (B) Measurement of chlorophyll content in <i>OsSWEET5</i>-overexpressing lines and WT plants. The samples were from the second leaves in (A). The results shown are the means of three independent measurements. Significant differences are calculated by <i>t</i>-test and shown by asterisks. *, <i>P</i><0.05 or **, <i>P</i><0.01. FW, Fresh weight. (C) Northern blot analysis of <i>OsSWEET5</i>-overexpressing lines. RNA was extracted from the second leaves in (A). (D) RT-PCR analysis of <i>SGR</i> in <i>OsSWEET5</i>-overexpressing lines (OX1 and OX2) and WT plants. The first-strand cDNAs were prepared using RNAs extracted from the second leaves of OX1, OX2 and WT plants at three-leaf stage.