Effect of osmotic pressure on cell proliferation in melanoma.

<p>500/well were plated on 96 multiwells (IgR39 and IgR37). The day after plating the cells were submitted to different osmotic pressure (from 0.2 to 1 kPa) for 3 or 6 days. At the end of the incubation the cells were fixed with 50%TCA for 2 hours at 4C and air-dry at room temperature. Thus, the cells were incubated with 0.05% SBR solution for 30 minutes at room temperature and then quickly rinsed four times with 1% acidic acid to remove unbound dye. Finally, the protein-bound dye was solubized with 10 mM TRIS and OD was measured at 510 nm with microplate reader 550 (Bio-Rad). a) The growth of IgR39 (non-metastatic) cells is not affected by pressure after 3 days, b) but cells grow significantly less after 6 days. c) IgR37 (metastatic) cells are unaffected by pressure both after c) 3 or d) 6 days. Statistically significant results according to the KS test () are denoted with *.