Crb, but not the Crb complex members Sdt and <i>D</i>PATJ, is enriched in the BCs of the embryonic hindgut.

<p>(A–D″) Confocal microscope images of stage 15 wild-type embryos. (<b>A</b>) Dorsal view of a whole mount embryo stained with anti-Crb, showing apical localisation in the PCs (blue arrowhead) and strong enrichment in the BCs (white arrowhead) of the hindgut (hg). Anterior is left. (<b>B</b>) Cross section through the large intestine stained with anti-Crb to show strong apical accumulation of Crb in the BCs (white arrowheads) and SAR localisation in the PCs (blue arrowhead). (<b>C–D″</b>) Confocal microscope images of embryonic hindguts stained with anti-Crb (magenta in C, C″, D and D″), anti-Sdt (grey in C′ and C″) and anti-<i>D</i>PATJ (grey in D′ and D″) as well as anti-Dlg (green in C″ and D″). BCs (white arrowheads) accumulate Crb (C, C″, D, D″) but not Sdt (C′ and C″) or <i>D</i>PATJ (D′, and D″). PCs (C–D″, blue arrowheads) localise Crb (C, C″, D, D″), Sdt (C′ and C″) and <i>D</i>PATJ (D′ and D″) in the SAR. (<b>E</b>) Box Plot showing the fluorescence intensity of anti-Crb staining in PCs and BCs of stage 15 wild-type embryos. The line within the box represents the median value; the whiskers represent the maximum and minimum values; *** indicate p-value <0.001, assessed by two-sided Student's <i>t-</i>test.(<b>F, G</b>) Box Plot showing the Pearson's correlation coefficient of Crb and Sdt, <i>D</i>PATJ, <i>D</i>Lin-7, Baz, <i>D</i>Par6, aPKC and Dlg in PCs (F) and BCs (G) of stage 15 wild-type embryos. The line within the box represents the median value; the whiskers represent the maximum and minimum values. Note the difference in the scale of the Pearson's correlation coefficient in F and G.