Immunohistochemistry of VEGF, VEGFR1 and VEGFR2 expression on cornea of all groups.

<p>(A) On normal cornea, VEGF, VEGFR1 and VEGFR2 were found to be presented on corneal epithelium. (B) In no treatment group, VEGF, VEGFR1 and VEGFR2 staining were found on corneal epithelium and corneal NV with co-localization with CD31 on week 1 and week 2. (C) In early treatment group, VEGF, VEGFR1 and VEGFR2 stained only on corneal epithelium on week 1 and week 2 after bevacizumab injection. No staining of VEGF, VEGFR1 and VEGFR2 on corneal vessels was found. (D) In late treatment group, staining of VEGF, VEGFR1 and VEGFR2 was found on corneal epithelium with co-localization with CD31 at 1 and 2 weeks after bevacizumab injection. Red: CD31; Green: VEGF, VEGFR1 and VEGFR2; Blue: Hoechst33258 which stains nucleus. Scale bar: 200 μm. Three repeats were performed to ensure consistent results. In early and late treatment groups: Week 1, 2 = Week 1, 2 after bevacizumab injection.