Effects on global translation and lambda light chain synthesis.

<p><b>A</b>) AHA incorporation is tested in MM cells stimulated with IL-6 for 30, 60, 120 or 180 mins. In <b>B</b>), AHA incorporation is tested in IL-6-stimulated EV-, WT or mutant SA eIF-4E-transfected MM cells. In <b>C</b>), EV or SA mutant-expressing cells are pre-treated +/− pp242, followed by IL-6. Bar graphs under panels are means+/−SD of 4 separate experiments. * denotes statistically significant increase in AHA incorporation induced by IL-6 in A and B and decrease versus IL-6-stimulated control in C). <b>D</b>) Lambda light chain ELISA assay in MM cell lysates, comparing EV- vs mutant eIF-4E-transfected cells +/− IL-6 and +/− pp242. Data are means+/−SD, n = 4. * denotes statistically significant difference from corresponding controls.