Inbreeding levels and demographic patterns in the Caucasian wolves inferred from genome-wide SNP data, in comparison with European wolf populations analysed in Pilot et al. [15].

<p>(A) Frequency distribution of runs of homozygosity (ROH). This figure has different scales in the vertical axis for the Caucasus (left, marked in red) and other populations (right) because of differences in the number of SNPs analysed; (B) Extent of linkage disequilibrium, represented as changes in an average genotypic association coefficient <i>r²</i> with an increasing inter-SNP distance. (C) Temporal changes of effective population size (<i>N_E</i>). We present the data for the populations representing extremes of the range (see Figure 5 in <a href="http://www.plosone.org/article/info:doi/10.1371/journal.pone.0093828#pone.0093828-Pilot1" target="_blank">[15]</a>), as well as the data for the Balkan and Spanish populations that were compared with the Caucasian population in other parts of this study. The Carpathian population is only presented in part A, because it had extreme values of ROH, but average values for other parameters.