Single-cell impedance spectroscopy: maximum length sequence analysis

A novel broadband high-speed impedance spectrometer has been developed for the analysis of single biological particles in a high-throughput microfluidic cytometer. The technique is based on obtaining the impulse response of the system using maximum length sequences (MLS) as the excitation signal. The impulse response is converted into the frequency domain using Fast Fourier Transform (FFT). Theoretical modeling and simulation of a single cell suspended in the cytometer show that the MLS technique is capable of high precision single particle analysis