PDI and ERp57 appear at the surface of NSDV-infected cells.

<p><b>A.</b> Samples were prepared as for <a href="http://www.plosone.org/article/info:doi/10.1371/journal.pone.0094656#pone-0094656-g001" target="_blank">Figure 1</a> except that, after fixing with 3% PFA, cells were labelled with mouse anti-PDI (clone 1D3; a–c), mouse anti-PDI (clone RL90; d–f) or rabbit anti-ERp57 (g–i). Then cells were again fixed with 3% PFA, opened with ice-cold methanol, and stained with rabbit antiserum against the viral N protein (a–f) or with mouse anti-PreGn antibody (g–i). Proteins were visualised by co-staining with Alexa Fluor 488 goat anti-mouse IgG (green) and Alexa Fluor 568 goat anti-rabbit IgG (red). <b>B.</b> Vero cells were infected with the NSDVi isolate at a MOI of 6 TCID₅₀ or left uninfected. After 16 h, cells were fixed using 3% PFA and left non-permeabilised. Cells were incubated with mouse anti-PDI (clone 1D3) antibody followed by Alexa Fluor 488 goat anti-mouse IgG (green). Nuclei were counterstained using DAPI (blue). Bars correspond to 40 μm.