The replication of NSDV induces secretion of PDI and ERp57 from infected cells.

<p>Vero cells were infected with the NSDVi isolate at a MOI of 0.1 TCID₅₀ or left uninfected. After 48 h, supernatants and cells were harvested; proteins were separated on acrylamide SDS-PAGE gels and cellular and viral proteins were detected by Western blotting using specific antibodies to PDI (clone RL90), ERp57, the viral N protein, calnexin (CNX) or α-tubulin.