DOX-PCL₆₃-b-PNVP₉₀ hinders colony formation in DL.

<p>Clonogenic assay was performed in six-well plates following seeding of 100 cells with clones produced by DL tumor cells. Untreated controls and PCL₆₃-b-PNVP₉₀ treated cells formed colonies (A and C). Smaller size colonies with less number of cells were observed following doxorubicin (0.5 μM) treatment (B). Cells treated with DOX-PCL₆₃-b-PNVP₉₀ treatment (0.5 μM) unable to form colony (D). Survival analysis of DL cells treated with free DOX (Red Line) or, DOX-PCL₆₃-b-PNVP₉₀ (Blue line), (p = 0.01) (E). DOX-PCL₆₃-b-PNVP₉₀ induced growth inhibition in DL cells is caspase dependent. Growth inhibition study in presence and absence of pan caspase inhibitor ZVAD-FMK for 48 h (n = 3, mean ±SD). MTT assay was performed as described above (G).Data presented as mean ± SD, n = 3.