<i>Semiquantitative</i> RT-PCR analysis of glaucoma-associated ECM components in ONH astrocytes after treatment with OPN.

<p>Treatment with 250/ml OPN for 72 hours has no effect on gene expression of glaucoma-associated ECM components (A, B), genes of the ECM degradation system (C–F), as well as stress response genes (G, H) in cultivated ONH astrocytes compared to controls (0 OPN). (A, C, E and G) Representative RT-PCR results upon treatment with 250 ng/ml OPN () and controls (0 OPN). (B, D, F and H) Densitometric analysis of <i>sq</i> RT-PCR results normalized to GAPDH. Values represent mean ± SD of 11 independent experiments (n = 11).