ERK activation is sustained in de novo RRV infection.

<p>(<b>A</b>) Quantitative immunoblots of uninfected (UI; left panels), UV irradiated-RRV infected (UV-RRV; MOI of 10 pre UV treatment; middle panels), or RRV-infected RhF (MOI of 10; right panels). 10 ug of cell lysate (10–15% of total lysate) was loaded per lane. Blots were probed with the following antibodies: anti-phosphorylated ERK (pERK), anti-Ran (loading control for cellular extracts), and anti-ERK1/2. (<b>B</b>) Graphical representation of the fold change in pERK2 over time relative to time zero. pERK2 values were normalized to Ran to correct for loading differences. Normalized pERK2 values were compared to time zero for each of the three conditions to determine the fold change. Two representative experiments are plotted; solid and dashed lines symbolize two separate experiments. Green, blue, and red lines represent UI, UV-RRV, and RRV respectively.