Alignment for GpsB and DivIVA proteins from several bacteria.

<p>(A) Multiple sequence alignments of GpsB and DivIVA sequences from streptococci and Gram-positive bacteria. Protein sequences similar to that of pneumococcus GpsB and DivIVA were identified by BLAST searches and aligned using CLUSTALW. Spn: <i>S. pneumoniae</i>; Sag: <i>S. agalactiae</i>, Bsu: <i>B. subtilis</i>; Sta: <i>S. aureus</i>, Mtb: <i>M. tuberculosis</i>; Sco: <i>S. coelicolor</i>. Yellow highlights the potential coiled-coil motifs retrieved from UniProtKB/Swiss-Prot:Q8CWP9 and UniProtKB/Swiss-Prot:C1CIN3 entry annotations for Spn-DivIVA (residues 34–135 and 199–236) and Spn-GpsB (36–63) respectively. The PF05103 PFAM DivIVA family signatures are mapped as green open boxes for DivIVA and GpsB. When identified, phosphorylation sites are red boxed. The <i>S. coelicolor</i> DivIVA phosphopeptide containing unidentified phosphorylation sites are highlighted in orange letters. Identical residues are in pink letters and positions showing conservation of similar residues are in blue. Dots indicate gaps introduced in sequences during alignment computation. The figure was rendered with the ESPript server <a href="http://www.plosgenetics.org/article/info:doi/10.1371/journal.pgen.1004275#pgen.1004275-Gouet1" target="_blank">[54]</a>. (B) Multiple sequence alignments of GpsB sequences from streptococci. Protein sequences were aligned using CLUSTALW. Spy: <i>S. pyogenes</i>; Sag: <i>S. agalactiae</i>, Smu: <i>S. mutans</i>; Sth: <i>S. thermophylus</i>; Ssa: <i>S. salivarius</i>; Spn: <i>S. pneumoniae</i>; Smi: <i>S. mitis</i>, Sgo: <i>S. gordonii</i>. The PFAM PF05103 DivIVA family signatures are mapped as green boxes. Yellow highlights the potential coiled-coil motifs retrieved from UniProtKB/Swiss-Prot:C1CIN3 entry annotations for Spn-GpsB (36–63). The phosphothreonine identified for <i>S. agalactiae</i> GpsB is red boxed. Glutamic acids possibly mimicking threonine phosphorylation are black boxed with white letters. Identical residues are in pink letters and positions showing conservation of similar residues are in blue. Dots indicate gaps introduced in sequences during alignment computation. The figure was rendered with the ESPript server <a href="http://www.plosgenetics.org/article/info:doi/10.1371/journal.pgen.1004275#pgen.1004275-Gouet1" target="_blank">[54]</a>.