<p>Phase contrast microscopy (left panel) and BADA labeling of PG (middle panel) images of WT, Δ<i>divIVA</i>, Δ<i>gpsB</i> and Δ<i>divIVA</i>Δ<i>gpsB</i> exponentially growing cells pulsed with BADA 4 min each at 37°C in THY medium. Overlay between phase contrast (red) and BADA (green) labeling is shown. Arrows show helical organization of PG synthesis. Scale bar, 5 µm.</p
A. Micrographs of PBP1a depletion ΔchvI with (+PBP1A) or without (-PBP1A) IPTG. Cells were grown to ...
<p>(A) Localization of GltB-mCherry, GltA-mCherry GltC-mCherry in the indicated genetic backgrounds....
Supplementary Video 1. Video shows a complete view across the Z-axis for GFP (left) and JPH2 (middle...
<p>(A) DivIVA-GFP localization in WT and Δ<i>gpsB</i> cells. Phase contrast (left), GFP fluorescent ...
<p>(A) Phase contrast microscopy (lower panel) and FM4–64 membrane staining (upper panel) images of ...
<p>Chromosomal copy of either <i>pbp2x</i>, <i>pbp2b</i>, <i>ftsW</i> or <i>rodA</i> were substitute...
<p>(A) FtsZ localization in WT, Δ<i>divIVA</i>, Δ<i>gpsB</i> and Δ<i>divIVA</i>Δ<i>gpsB</i> cells. P...
<p>(A) Western immunoblot of whole-cell lysates from the wild type (WT), Δ<i>stkP</i>, <i>gpsB::gfp-...
(A) Fluorescence microscopy images of exponentially growing Rm2011 rgsP-egfp (gene fusion at the nat...
<p>(A) Localization of MglA-YFP in WT, <i>romR</i> mutant and <i>romR mglB</i> mutant. (B) Localizat...
<p>(<b>A</b>) <i>act15::PakD-GFP</i> cells were grown in glass chamber slides overnight, then fixed ...
<p><b>A.</b> In <i>rga1Δ</i> cells (HPY2204), Gic2-PBD-RFP localized continuously to (a) the proxima...
<p><b>A</b> representative single cell image series of Rvs167-GFP distribution at different stages o...
Supplementary Figure 1. Video shows a complete view across the Z-axis for GFP (left) and JPH2 (middl...
<p>(A) A representative brightfield microscopy image of protoplasts from <i>B. subtilis</i> PE103 co...
A. Micrographs of PBP1a depletion ΔchvI with (+PBP1A) or without (-PBP1A) IPTG. Cells were grown to ...
<p>(A) Localization of GltB-mCherry, GltA-mCherry GltC-mCherry in the indicated genetic backgrounds....
Supplementary Video 1. Video shows a complete view across the Z-axis for GFP (left) and JPH2 (middle...
<p>(A) DivIVA-GFP localization in WT and Δ<i>gpsB</i> cells. Phase contrast (left), GFP fluorescent ...
<p>(A) Phase contrast microscopy (lower panel) and FM4–64 membrane staining (upper panel) images of ...
<p>Chromosomal copy of either <i>pbp2x</i>, <i>pbp2b</i>, <i>ftsW</i> or <i>rodA</i> were substitute...
<p>(A) FtsZ localization in WT, Δ<i>divIVA</i>, Δ<i>gpsB</i> and Δ<i>divIVA</i>Δ<i>gpsB</i> cells. P...
<p>(A) Western immunoblot of whole-cell lysates from the wild type (WT), Δ<i>stkP</i>, <i>gpsB::gfp-...
(A) Fluorescence microscopy images of exponentially growing Rm2011 rgsP-egfp (gene fusion at the nat...
<p>(A) Localization of MglA-YFP in WT, <i>romR</i> mutant and <i>romR mglB</i> mutant. (B) Localizat...
<p>(<b>A</b>) <i>act15::PakD-GFP</i> cells were grown in glass chamber slides overnight, then fixed ...
<p><b>A.</b> In <i>rga1Δ</i> cells (HPY2204), Gic2-PBD-RFP localized continuously to (a) the proxima...
<p><b>A</b> representative single cell image series of Rvs167-GFP distribution at different stages o...
Supplementary Figure 1. Video shows a complete view across the Z-axis for GFP (left) and JPH2 (middl...
<p>(A) A representative brightfield microscopy image of protoplasts from <i>B. subtilis</i> PE103 co...
A. Micrographs of PBP1a depletion ΔchvI with (+PBP1A) or without (-PBP1A) IPTG. Cells were grown to ...
<p>(A) Localization of GltB-mCherry, GltA-mCherry GltC-mCherry in the indicated genetic backgrounds....
Supplementary Video 1. Video shows a complete view across the Z-axis for GFP (left) and JPH2 (middle...