QKI regulates cancer-associated splicing changes.

<p>(A) Western blot analysis of QKI-5 expression in BEAS2B cells stably expressing control (sh-Luc) and two QKI shRNAs (sh-Q1 and sh-Q3). (B) RNA-Seq analysis detected 799 events with alternative splicing changes after QKI was knocked down (Benjamini-Hochberg adjusted <i>P</i>-value<0.05). (C) RT-PCR analysis of the splicing patterns of QKI targets in control- and QKI-knockdown BEAS2B cells. The average percentages of exon inclusion with standard deviations are shown below (n = 3). (D) Down-regulation of QKI in NSCLC causes cancer-associated splicing events. Fold changes of exon inclusion in tumor tissue normalized to that in normal tissue are shown below the representative RT-PCR analyses. A1–A5: adenocarcinoma patient samples. S1–S4: squamous cell carcinoma patient samples. Among the 9 paired samples, the expression levels of QKI-5 protein normalized to GAPDH are down-regulated in all paired samples except A5 patient samples as determined by Western blotting. T: tumor tissue; N: adjacent normal tissue.