Peptide-fusion protein uptake and real time cell proliferation assay.

<p>(A) Cellular uptake of the peptide-fusion protein was analyzed by immunostaining images that show localization of peptide-fusion protein around cells nuclei. (B) Cell proliferation was measured using xCELLigence Real-Time Cellular Analysis (RTCA) system. Cell viability and growth was monitored continuously after incubating the cells for 18 h at standard condition and applying increased concentrations of the PG1-MAP30-PLSN protein (6.25, 12.5, 25, 50, 100, 200 and 400 μg/ml or 0.17, 0.34, 0.67, 1.35, 2.70, 5.40 and 10.81 μM).The effects of the peptide-fusion protein on cell proliferation were insignificant at the doses less than 25 μg/ml (0.67 μM) for 82 h as the cell index was similar with untreated control cells. Cell proliferation was considerably decreased at the dose more than 50 μg/ml (1.35 μM).