The RGS domain of Axin is not necessary for colocalisation with APC or APCL.

<p>DLD1 cells were transiently transfected on day 1 with the indicated APC (<b>A</b>) or APCL (<b>B</b>) constructs or with the indicated N-terminal myc-tagged Axin constructs, either individually or in combination. The cells were stained on day 3 with an anti-myc antibody and Hoechst dye. Cells transfected with yAPCL alone displayed dotty and fibre-like expression patterns, with yAPC alone exhibited a fibre-like expression pattern, with myc-Axin alone displayed diffuse and dotty expression patterns and with myc-Axin(298–832) alone exhibited only a dotty expression pattern. Coexpression of myc-Axin and APC or APCL resulted in colocalisation, which was also observed with when the RGS domain (298–832) was deleted from Axin. The percentages indicate the proportion of different localisation patterns in the transfected cells. The imaging parameters were identical for the myc constructs; the yAPCL signal was more intense than the yAPC signal. Bar, 10 μM.