Early repression of LC3 recruitment is independent of IcsA.

<p>(A) LC3 recruitment to the vicinity of wild-type (WT), Δ<i>icsB</i>, or <i>icsA</i> Δ<i>icsB S. flexneri</i>, complemented or not with pIcsB-flag, at 40 min. of infection of HeLa cells that had been transfected with LC3-GFP. (B) Representative images corresponding to (A). Arrowheads, bacteria with LC3 recruitment. (C) Toca-1 recruitment to <i>icsA S. flexneri</i> at 40 min. of infection of HeLa cells that had been transfected with Myc-Toca-1, with immunofluorescence using antibody to Myc and staining with DAPI. (D) Cells containing LC3 encircling WT, Δ<i>icsB</i>, <i>icsA</i>, or <i>icsA</i> Δ<i>icsB S. flexneri</i>, complemented or not with pIcsB-flag, at 4 hr of infection of MEFs that had been transfected with LC3-GFP. (E) Distinctive LC3 distribution patterns around WT <i>S. flexneri</i> at early (40 min., top panels) and late (4 hr., bottom panels) times after infection. Data represent the mean ± S.D. of three independent experiments. *P<0.05. Images are representative. †, each bar was statistically different from bars for isogenic strain that contains <i>icsA</i>. Scale bars, 5 µm.