Post embedment immunostaining of p-Tau (Ser396) with 18 nm particles gold-conjugated antibody.

<p>(<b>A</b>) Control PC12 cells. (<b>B1–B3</b>) PC12 cells after 48 h treatment with 10 μM ASN. (<b>C</b>) Bar charts representing the number of gold particles representing p-Tau (Ser396) protein. Gold particles are indicated by arrows. ASN treatment resulted in significantly elevated expression of p-Tau protein in comparison to the control PC12 cells. Data represent the mean value ± S.E.M. for 3 independent experiments. *** p<0.001 <i>versus</i> control using a Student's <i>t</i>-test. Bar 1 μm. (N - nucleus, M - mitochondria, ER - endoplasmic reticulum, E - endosomes).